Composite

Part:BBa_K2505009:Design

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)


Ptra-rbs-gfp-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 821


Design Notes

This part produces GFP induced by 3OC8AHL molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8).

Source

Ptra was derived from agrobacterium.

References

C Fuqua, S C Winans. (1996) Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 178: 435–440.